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Co-localization of Bmi1 siRNA and DOX in KB cells. KB cells grown in a monolayer were incubated with free DOX, DOX/siRNA-L, FA-DOX/siRNA-L and for 1 h at 37°C. For folate blocking group, 1 mM free folate was added to the incubation media prior to the addition of FA-DOX/siRNA-L. Bmi1 siRNA was labeled with FAM (Green) and DOX emits red fluorescence by itself. The nucleus was counterstained with DAPI (Blue).
