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MurM decreases production of alanylated tRNALys anticodons CTT (A) and TTT (B) by LysRS. Misaminoacylation time courses for generation of [3H]Ala-tRNALys by 2 µM active pneumococcal LysRS in the presence of 0.6 µM MurM are shown. The concentration of wild-type pneumococcal tRNALys used was 7 µM. The concentration of [3H]Ala used was 250 µM. Data sets are the averages of three independent experiments. Error bars indicate standard errors.
