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Comparative S. pneumoniae and E. coli IleRS-catalyzed mischarging of cognate tRNAIle with leucine (A) or valine (B). Aminoacylation time courses were evaluated in the presence of 200 µM l-[14C]Leu (A) or 200 µM l-[14C]Val (B) for 1 µM active pneumococcal or E. coli IleRS. Wild-type pneumococcal or E. coli tRNAIle (anticodon GAT) was used at a concentration of 10 µM. The presented data set is the average of three independent experiments. Error bars show standard errors.
