FIG 8 .
Comparative S. pneumoniae IleRS-catalyzed mischarging of wild-type and G16C tRNAIle with isoleucine (A), leucine (B), or valine (C). For the cognate amino acid, aminoacylation time courses were carried out in the presence of 22 µM l-[14C]Ile and 500 nM active pneumococcal IleRS (A). For the noncognate amino acids, aminoacylation time courses were carried out in the presence of 200 µM l-[14C]Leu or l-[14C]Val for 1 µM active pneumococcal IleRS. Wild-type or G16C pneumococcal tRNAIle (anticodon GAT) was used at a concentration of 10 µM. The presented data set is the average of three independent experiments. Error bars show standard errors.