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Deacylation of mischarged pneumococcal Leu-tRNAIle by S. pneumoniae and E. coli IleRS. Deacylation time courses were carried out as described above by incubation of 50 pM [14C]Leu-tRNAIle with 0.5 µM pneumococcal (A) or E. coli (B) IleRS. For the control reaction mixture, an equal volume of protein storage buffer was added. Error bars indicate standard errors.
