. 2014 Sep 9;5(5):e01656-14. doi: 10.1128/mBio.01656-14

TABLE 1 .

Kinetic parameters for aminoacylation of tRNA^Ile with Ile and Leu by pneumococcal IleRS^a

Amino acid	K_m (μM)	V_max (μM/min/mg)	k_cat (min⁻¹)	k_cat/K_m	Specificity constant
Ile	0.4 ± 0.1	0.0005 ± 0.0002	0.06 ± 0.02	0.14	1:2,850
Leu	3,200 ± 290	0.0015 ± 0.0002	0.16 ± 0.02	0.000049

^{^a}

Means and standard errors are shown. V_max and k_cat values were determined using protein concentration estimations obtained via the Bradford assay.