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. 2014 Aug 26;5(5):e01684-14. doi: 10.1128/mBio.01684-14

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Copyright © 2014 Park and Kiley.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 5 — Phosphorylation dependence of ArcA repression of P_1icdA in strains with strengthened ArcA binding sites. The fold repression of P_1icdA-lacZ in strains containing arcA-FRT-cat-FRT (white bars), arcA(D54A)-FRT-cat-FRT (light-gray bars), or arcA(D54E)-FRT-cat-FRT (dark-gray bars) was determined from cells grown under anaerobic (A) or aerobic (B) conditions and calculated by dividing the β-galactosidase activity of a ΔarcA strain by the activity with each of the arcA alleles. Asterisks denote DR elements that have been mutated toward the consensus. Error bars represent the standard errors of results from at least three independent replicates.