Figure 4.

Lung slices of the murine cardiac lobe examined by light microscopy. After mounting on a frame slide, the sections were visualized without counter-stain at 10× (A,C,D) and 20× (B) magnification. The respiratory bronchiole and alveolar ducts were readily identified (B). The relevant anatomic structure was demarcated by software annotation (C) and laser microdissected using computer control (D). Successful dissection is demonstrated as the structure drops into the frame slide and out of the optical plane (D). Bar = 1 mm.