Fig. 2.

Effect of dietary OTC supplementation on hepatic GSH content. Young and aged C57Bl6 mice were placed on either a 0.5% OTC or a SAA def. control diet for 4 weeks. Hepatic GSH (A) and GSH/GSSG (B) contents were measured in whole liver homogenates prepared from individual animals (n = 3–4 per group) using a Bioxytech® GSH/GSSG-412™ assay kit. Data are shown as mean values ± SD. Statistical analyses were done by two-way ANOVA. The main effects of the diet and age are indicated with an asterisk (*P < 0.05). No interaction between both factors was present.