Fig. 4.

25-HC is a highly specific trigger for accumulation of esterified oxysterol and activation of ER stress in Nceh1-deficient TGEMs. A, B: TGEMs were incubated with various kinds of oxysterols (10 μg/ml) in the presence or absence of CS-505 (10 μM) for 12 h. Lipids were separated by thin layer chromatography (A), and the expression profile was examined (B). s, spliced; u, unspliced; Chol, cholesterol. Oxysterols are abbreviated as follows: 5α6α, 5α6α-epoxycholesterol; 7β, 7β-hydroxycholesterol. Values above each band/spot indicate the fold difference evaluated by densitometry. Xbp-1 splicing was assessed as the percent spliced Xbp-1[spliced Xbp-1/(spliced XBbp-1 plus unspliced Xbp-1)].