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. 2014 Oct;55(10):2113–2123. doi: 10.1194/jlr.M051755

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Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 6. — RP-HPLC/MS² analysis of oxygenation products of 18:2n-6 formed by recombinant FOXB_03425 and transformation of 10-HPODE by this enzyme. A: RP-HPLC/MS² analysis revealed biosynthesis of 8- and 10-H(P)ODE, and 12(13)-epoxy-10-hydroxy-(8E)-octadecenoic acid (peak I) from 18:2n-6. B: The top chromatogram shows transformation of 10R-HPODE by FOXB_03425 to 12(13)-epoxy-10(R)-hydroxy-(8E)-octadecenoic acid (peak I), and the bottom chromatogram shows that 10S-HPODE was a poor substrate.