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Enzymatic conversion of 18:2n-6 by recombinant 10R-DOX-EAS. Abstraction of the pro-S hydrogen of C-8 is followed by antarafacial oxygen insertion mainly at C-10 and to some extent at C-8, yielding 10R- and 8R-HPODE, respectively. 10R-HPODE is further converted by the EAS activity to 12S(13R)-epoxy-10(R)-hydroxy-8(E)-octadecenoic acid by heterolytic scission of the O-O bond with formation of P450 compound I in a process supported by Asn965 (cf. Fig. 4).
