TABLE 3.
Intra-day and inter-day precision of determination method for lysophospholipids
(μM) | (pmol) | LPC (14:0) | LPC (16:0) | LPC (18:0) | LPC (18:1) | LPA (18:1) | LPE (18:1) | LPG (18:1) | LPI (18:1) | LysoPS (18:1) |
Intra-day precision (%) (n = 4) | ||||||||||
0.01 | 0.1 | 9.1 | 0.7 | 1.0 | 2.6 | 13.8 | — | 3.9 | 9.7 | 12.6 |
0.05 | 0.5 | 2.3 | 3.3 | 2.0 | 0.9 | 10.7 | — | 11.0 | 2.1 | 6.8 |
0.1 | 1 | 2.4 | 2.5 | 1.8 | 2.8 | 3.2 | — | 2.8 | 5.1 | 4.5 |
0.5 | 5 | 0.6 | 0.5 | 1.5 | 1.6 | 3.1 | 10.1 | 0.3 | 3.9 | 5.1 |
1 | 10 | 1.6 | 0.4 | 0.4 | 1.9 | 4.2 | 3.9 | 1.1 | 3.1 | 5.3 |
5 | 50 | 2.0 | 2.0 | 0.9 | 1.4 | 2.7 | 3.9 | 4.4 | 3.4 | 6.0 |
10 | 100 | 1.2 | 0.6 | 1.6 | 0.5 | 1.7 | 1.6 | 1.0 | 3.1 | 1.7 |
50 | 500 | 2.1 | 3.6 | 2.8 | 3.2 | — | — | — | — | — |
Inter-day precision (%) (n = 4) | ||||||||||
0.01 | 0.1 | 6.9 | 3.4 | 5.4 | 11.1 | 5.6 | — | 9.1 | 10.8 | 39.3 |
0.05 | 0.5 | 0.1 | 3.8 | 2.5 | 0.2 | 1.4 | — | 7.6 | 3.5 | 6.4 |
0.1 | 1 | 0.9 | 3.9 | 2.0 | 3.5 | 2.6 | — | 4.6 | 8.1 | 12.8 |
0.5 | 5 | 4.1 | 0.7 | 0.5 | 1.7 | 1.4 | 8.9 | 0.8 | 1.8 | 3.5 |
1 | 10 | 4.8 | 2.2 | 0.2 | 0.4 | 4.6 | 1.0 | 3.0 | 1.2 | 7.8 |
5 | 50 | 1.8 | 1.5 | 0.6 | 0.5 | 1.3 | 2.2 | 2.3 | 1.4 | 1.3 |
10 | 100 | 1.8 | 2.3 | 2.9 | 2.5 | 0.8 | 0.8 | 0.4 | 1.6 | 3.3 |
50 | 500 | 3.8 | 5.2 | 4.8 | 6.5 | — | — | — | — | — |