Table 2. Characterization of Four Engineered Nanomaterial (ENM) Dispersions (at 50 μg/mL) in RPMI with 10% FBS Cell Culture Medium Obtained from Dynamic Light Scatteringa and Tunable Resistive Pulse Sensing.
dynamic light scattering (DLS) |
tunable resistive pulse sensing (TRPS) |
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ENM type | dh,z-ave (nm) | PdI | ζ (mV) | peak size | size mean (nm) | size mode (nm) | range (nm) | concentrationb (#/mL) | fraction of total number |
SWCNH-ox | 261 ± 3 | 0.23 | –9.3 ± 1.0 | small | 249 | 212 | 177–572 | 2.70 × 1010 | 0.844 |
large | 660 | 684 | 572–877 | 5.00 × 1009 | 0.156 | ||||
Printex- 90 | 270 ± 8 | 0.37 | –13.1 ± 1.1 | small | 264 | 198 | 173–717 | 9.80 × 1007 | 0.971 |
large | 1068 | 860 | 717–1476 | 2.90 × 1006 | 0.029 | ||||
CeO2 | 265 ± 2 | 0.31 | –12.1 ± 0.2 | small | 268 | 211 | 183–659 | 1.80 × 1008 | 0.987 |
large | 1066 | 847 | 659–1922 | 2.60 × 1006 | 0.013 | ||||
Ni Inco | 234 ± 7 | 0.40 | –14.4 ± 1.5 | small | 268 | 199 | 176–715 | 8.80 × 1007 | 0.990 |
large | 1136 | 904 | 715–1897 | 4.10 × 1006 | 0.010 |
dh,z-ave, hydrodynamic diameter; PdI, polydispersity index, a measure of the broadness of size distribution; ζ, Zeta Potential, a measure of surface charge in the cell culture medium.
Concentration for SWCNH-ox determined at 5 μg/mL.