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. 2014 Sep 24;8:317. doi: 10.3389/fnbeh.2014.00317

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Copyright © 2014 Prast, Schardl, Schwarzer, Dechant, Saria and Zernig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colocalization of EGR1 with the neuronal marker NeuN but not with glial markers. EGR1 immunoreactivity is shown in green, all other markers are shown in red. Colocalization of immunoreactivity was found only in cells positive for the neuronal nuclear protein NeuN (A). No colocalization with EGR1 was observed in glial cells. The employed glial markers were glial fibrillary acidic protein for astrocytes (B), myelin basic protein for oligodendrocytes (C), and tomato lectin for microglia (D). Images were taken with a laser scanning confocal microscope with a magnification of 100× (bar size, 10 μm).