Table 1.
GC quantification of FAME content of adaptive evolved strains of CC124 and sta6-1 at different stages
| Strain | Different stages (Day) | Nitrogen-repletion conditions | Nitrogen-depletion conditions | Fatty acid (% of total fatty acids in nitrogen-depletion conditions) a | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total lipid (%) | Total lipid (%) | C14:0 | C14:1 | C15:0 | C15:1 | C16:0 | C16:1 | C17:1 | C18:0 | C18:1-3 (n) b | ||
| CC124 | 19 | 6.6 | 10.5 | 0.3 | 0.2 | 0.2 | 0.3 | 39.3 | 2.7 | 2.7 | 5.4 | 45.3 |
| 32 | 5.9 | 13.5 | 0.3 | 0.0 | 0.2 | 0.2 | 40.1 | 4.5 | 2.8 | 3.4 | 45.3 | |
| 62 | 5.3 | 11.4 | 0.2 | 0.0 | 0.2 | 0.4 | 51.7 | 0.7 | 2.0 | 8.5 | 34.8 | |
| 77 | 7.0 | 15.9 | 0.2 | 0.0 | 0.2 | 0.4 | 40.7 | 0.5 | 1.2 | 8.9 | 46.4 | |
| sta6-1 | 14 | 5.8 | 5.9 | 0.4 | 2.0 | 0.6 | 0.8 | 85.3 | 2.7 | 0.7 | 5.2 | 45.0 |
| 27 | 6.7 | 8.1 | 1.1 | 0.0 | 0.5 | 0.7 | 40.6 | 1.2 | 1.8 | 5.0 | 47.0 | |
| 43 | 7.4 | 14.0 | 0.2 | 0.0 | 0.1 | 0.3 | 48.9 | 0.6 | 2.4 | 11.3 | 35.8 | |
aAll data are expressed as mean quantity of triplicate experiments.
bC18:1n9c, C18:2n6t, C18:3n6c. Abbreviations: GC, Gas chromatography; FAME, Fatty acid methyl esters.