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. Author manuscript; available in PMC: 2014 Sep 25.
Published in final edited form as: J Biomol NMR. 2011 Jul 12;50(4):421–430. doi: 10.1007/s10858-011-9528-y

Figure 5.

Figure 5

15N-HSQCs of uniformly 15N-labeled MBP in aqueous solution and in CTAB/hexanol reverse micelles in various bulk alkane solvents. No deuteration or TROSY effect was employed. Spectra were collected with identical parameters (128 complex 15N increments) and were processed identically except that the aqueous spectrum was collected with 64 scans while all others were collected with 16 scans. All spectra were recorded at 500 MHz (1H) and 25 °C. The propane and ethane samples were pressurized to 3000 and 4500 psi, respectively. The improved tumbling behavior shown in Figure 4 with reduced viscosity is manifested here as improved resolution and signal-to-noise.