Table 2.

Class I MHC-restricted peptides used to detect de novo CD8⁺ T cell response by IFN-γ direct ELIspot assay

Allele	Sequence	Epitope
HLA-A1	DAEKSDICTDEY [27]	Tyrosinasea240–251S
	SSDYVIPIGTY [28]	Tyrosinase146–156
	EADPTGHSY [29]	MAGE-A1161–169
	EVDPIGHLY [30, 31]	MAGE-A3168–176
HLA-A2	YMDGTMSQV [32]	Tyrosinaseb369–377D
	IMDQVPFSV [33]	gp100c209–217-2M
	YLEPGPVTA [34]	gp100280–288
	GLYDGMEHL [35]	MAGE-A10254–262
	AAGIGILTV [36]	MART-1/MelanA27–35
	SLLMWQITA [37]	NY-ESO-1d157–165
HLA-A3	ALLAVGATK [38]	gp10017–25
	LIYRRRLMK [28]	gp100614–622
	SLFRAVITK [26]	MAGE-A196–104
	ASGPGGGAPR [39, 40]	NY-ESO-153–62

^aSubstitution of S for C at residue 244

^bPost-translational change of N to D at residue 370

^c 209-2M, substitution of M for T at position 210

^dModified