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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Chem Biol. 2014 May 8;21(6):792–801. doi: 10.1016/j.chembiol.2014.03.012

Figure 1. Principles of Multicolor PFC.

Figure 1

(A) Schematic of the setup, together with signal traces in the green and red channels before, during, and after photoswitching (upper right inset) and a diagram of the alignment of the laser beams on the sample (lower right inset). CL, cylindrical lens; DM, dichroic mirror; F, bandpass filter; AL, achromatic lens; S, mechanical slit; PMT, photo-multiplier tube, and PC, a computer.

(B and C) The underlying principle (B) and PFC traces (C) of photoswitching and detection are depicted.