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. 2014 Oct 10;21(11):1591–1604. doi: 10.1089/ars.2013.5404

FIG. 3.

FIG. 3.

APCs express higher levels of anti-oxidant enzymes as compared with endothelial cells (ECs). (A) Differential expression of antioxidant enzymes in HUVECs and APCs, as assessed by gene array: Data are expressed as median and max-min values, ***p<1e-05. (B) Catalase and SODs mRNA levels in APCs, HUVECs, and SVECs, as assessed by quantitative PCR: Data are means±SEM of n=3 biological replicates, *p<0.05; **p<0.01 versus APCs. (C), Representative Western blot of Catalase and SODs. (D) Densitometry analysis and blot quantification: Data are means±SEM of n=3, *p<0.05 versus APCs. (E) Western blot showing SOD3 expression in APC and EC conditioned culture media (CCM). (F) Bar graph showing the quantification of SOD3 protein levels in CCM by densitometric analysis: Data are means±SEM of n=3, *p<0.05 versus APCs.