FIG. 7.

Oxidative stress does not impair APC-induced support of network formation by endothelial cells. (A–D) Representative images (A, C) and bar graphs (B, D) showing the network formation by HUVECs and SVECs alone (i, i’) or HUVECs and SVECs in coculture with APCs (ii, ii’). Network formation was strongly enhanced in coculture compared with HUVECs alone. APC preconditioning with H₂O₂ (250 μM, 24 h) did not impair the enhancement of network formation by APCs (iii, iii’). Values are expressed as cumulative tube length per field and means±SEM of three experiments performed in triplicate; *p<0.05 versus HUVECs or SVECs alone. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars