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Cd-Hfq cannot substitute for Ec-Hfq in the negative control of ptsG expression. (A) RO91-Δhfq bacteria transformed with the various plasmids were grown at 37°C on LB plates containing appropriate antibiotics and 0.2% α-methylglucoside. (B) Northern blots showing SgrS and ptsG transcripts detected in strains grown in LB after addition of 1% α-MG for 20 min. The strain was also transformed with pEc-HfqQ41S.
