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. 2014 Oct;20(10):1621–1631. doi: 10.1261/rna.045583.114

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© 2014 Lin et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — The phosphorylation status of RBM4 modulates its cellular localization and effect on alternative splicing events. MCF-7 cells were transiently transfected with an expression vector encoding wild-type RBM4, the S309A mutant (nonphosphorylatable; SA), or the S309D mutant (phosphomimetic; SD) for 24 h. (A) Localization of overexpressing RBM4 proteins was examined using indirect immunostaining with an anti-FLAG antibody. (B) Total RNA was isolated from the transfected MCF-7 cells and analyzed using RT-PCR, as described in Figure 3A. The cell lysates were subjected to an immunoblotting assay by using anti-FLAG and anti-GAPDH antibodies. The bar graph presents the relative ratio of IR-B and MCL-1_S in the transfected cells in three independent experiments. (***) P < 0.005.