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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Cancer Res. 2014 Jun 5;74(15):4183–4195. doi: 10.1158/0008-5472.CAN-14-0404

Figure 5. miR-128 regulates a cohort of self-renewal genes in PCa cells.

Figure 5

A. Predicted binding of miR-128 to the 3′-UTRs of BMI-1, NANOG, TGFβR1, EGFR, and E2F3.

B. qRT-PCR analysis of the effect of miR-128 overexpression on mRNA levels of the indicated genes in PC3, PPC-1, DU145 and LNCaP cells. Cells were transfected with either miR-128 or NC mimic (30 nM) for 48 h. RNA levels were normalized to GAPDH and data shown are relative to the corresponding NC control. Red lines were used as calibrators. Data represent the mean ± S.D from several independent measures. *P<0.05; **P<0.01.

C. Western blot analysis of the effect of miR-128 overexpression on protein levels of BMI-1, TGFBR1 and EGFR in indicated cells. Densitometric values relative to non-transfected (NT) cells were given below. GAPDH was used as a loading control.