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. 2014 Sep 24;9(9):e104666. doi: 10.1371/journal.pone.0104666

Figure 3. Dual-luciferase reporter assay validates that miR-377 directly targets VEGF.

Figure 3

(A): Computational miRNA target prediction analysis coincidentally reveals that the fragment 5′-GAAUCAC-3′ of miRNA-377 pairs well with the fragment 5′-GUGAUUC-3′ located at the 1568–1574 nt of VEGF 3′ UTR, which is a highly conserved site (red fonts) in most of mammals (e.g. rat, human, chimpanzee, rhesus, bushbaby, treeshrew, mouse). (B): Schematic diagram of Dual-Luciferase reporter vector (pEZX-MT01) carrying the VEGF3′ UTR. (C): Quantitative data for dual-luciferase reporter assay results. All values were expressed as means ± SE; n = 6 for each group. *P<0.05 was considered statistically significant.