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. 2014 Sep 5;5(8):670–678. doi: 10.7150/jca.9688

Figure 1.

Figure 1

EGF differentially affects cell viability in EGFR-expressing ovarian cancer cells. (A) Expression pattern of EGFR subtypes in a mouse and five human ovarian cancer cell lines. Whole cell lysates were prepared from each cell line and a western blot was carried out using antibodies specific to ErbB1, ErbB2, ErbB3 and ErbB4. Abbreviations used for ovarian cancer cell lines = SK (SKOV-3); OV (OVCAR-3); Ca (CaOV-3); TOV (TOV-21G); ID8 (ID8 mouse cells) and A (A2780). β-actin was used as a loading control. As positive controls for ErbB3 and ErbB4, MCF7 cells were used. MB231 cells are used as a positive control for ErbB1. (B) Differential effect of EGF on cell viability in various ovarian cancer cell lines. Cells were treated for 48 h with EGF (10 ng/mL). The cell viability assay was performed using MTT; values were normalized to untreated controls for each cell line. * indicates significance (p≤0.05) between EGF treated versus untreated cells by the paired Student's t-test. ** indicates significance (p≤0.05) between the cell lines having EGF-induced viability. Experiments were performed in triplicate and all data are shown as mean ± SEM.