Fig. 3.

Phlda2-induced fetal growth restriction is asymmetric (129 strain background). (A) Ratio of brain, lungs, heart, liver and kidney wet weights at E18.5 expressed as a proportion of body weight taken from samples described in Fig. 2. Brain weight was heavier as a proportion of body weight, compared with controls, whereas liver and kidney, sites of embryonic Phlda2 expression, were lighter. Normalising Phlda2 expression restored organ weight to non-transgenic proportions. (B) Ratio of brain as a proportion of body weight in E18.5 fetuses carrying three copies of the BAC transgene, Phlda2^{+/+BACx3(129)} (4×). (C) Fetal and postnatal weight data for the line Phlda2^{+/+BACx3(129)} (4×). Transgenic fetuses were lighter at birth but rapidly gained weight such that there was no significant difference in weight to their non-transgenic counterparts by P7. Numerical data is given in supplementary material Table S3. NS, not significant (P>0.05), *P<0.05, **P<0.01, ***P<0.005.