Table 3.
Secondary Antibodies.
| Antibody | Target | Working Dilution | Source |
|---|---|---|---|
| Goat Anti Rabbit, FITC | Ig H+L | 1:1000 | Sigma-Aldrich, Milano, Italy |
| Sheep anti Fluorescein Fab, Alkaline Phosphatase | FITC | 1:700 | Roche Applied Science; Indianapolis, IN |
| EnVision+ System - HRP Labelled Polymer Anti Mouse | Ig H+L | neat | Dako, Glostrup, Denmark |
| EnVision+ System - HRP Labelled Polymer Anti Rabbit | Ig H+L | neat | Dako |
| EnVision+ System - HRP Labelled Polymer Anti Mouse + Rabbit | Ig H+L | neat | Dako |
| Donkey Anti Rabbit Alkaline Phosphatase | Ig H+L | 1:1000 | Jackson ImmunoResearch Laboratories, West Grove, PA |
| Donkey Anti Rabbit Fab | Ig H+L | 1:100 | Rockland |
| Goat Anti Mouse, Biotin-SP | IgH γ1 | 1:500 | Jackson ImmunoResearch |
| Goat Anti-Mouse IgG3, Rhodamine Red-X | IgH γ3 | 1:500 | Jackson ImmunoResearch |
| Goat Anti-Mouse IgG2a, Rhodamine Red-X | IgH γ2a | 1:500 | Jackson ImmunoResearch |
| Goat Anti Mouse IgG2a, ATTO 488 | IgH γ2a | 1:200 | Rockland Immunochemicals; Gilbertsville, PA |
| Donkey Anti Rabbit, DyLight 549 | Ig H+L | 1:500 | Jackson ImmunoResearch |
| Goat Anti Mouse IgG1, ATTO 647N | IgH γ1 | 1:200 | Rockland |
| Donkey Anti Rabbit, Alexa Fluor 680 | Ig H+L | 1:100 | Invitrogen, Carlsbad, CA |
| Streptavidin, DyLight 488 | biotin | 1:500 | Jackson ImmunoResearch |