Figure 1.

The domain structure of FMNL1 isoforms and the regions used for antibody production and transfections. FMNL1 isoforms 1 and 2 show only minor variations in their C-terminal region. The C-terminus of isoform 3 differs from the others because of an intron retention, which partly changes the DAD domain and endows constitutive activation of this isoform. The antigenic sequence for antibody production is within the common FH2 domain. The construct used for transfections harbors most of the FH2 domain and the DAD domain of isoform 1.