Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Immunol. 2014 Jun 25;193(3):1373–1382. doi: 10.4049/jimmunol.1400145

Figure 4. Invasin-mediated dose dependent changes in IL-18 secretion.

Figure 4

A) Caco-2 cells were infected for 2 hours with JB580C or the JB580C inv mutant at the indicated MOI. After 2hrs, cells were treated with 25μM of the pore-forming toxin nigericin, and after 30 minutes, 20μg/ml of gentamycin to kill extracellular bacteria. ELISA determined the concentration of IL-18 in the conditioned media. Data represents mean ± SD of triplicate samples from two independent experiments. *p < 0.01; ** p < 0.001; ***p <0.0001. B) Caco-2 cells were infected with E. coli carrying an empty vector or expressing WT Y. enterocolitica invasin at the indicated MOI for 2hrs and then cells were treated with 25μM of nigericin. ELISA determined the concentration of IL-18 in the conditioned media. Data represents mean ± SD of three independent experiments. *p < 0.05; **p<0.01.