Figure 3.

Splenic atrophy is prevented with RTL1000. Spleens were harvested 96 h after MCAO. Splenocytes were isolated and counted by a Cellometer T4 and total splenocyte number was calculated (A). Values represent mean numbers (±s.e.m.) of 6 young vehicle, 5 young RTL, 12 older vehicle, and 13 older RTL mice. Splenocyte death was determined by 7-AAD fluorescence (B). Values represent mean numbers (±s.e.m.) of 5 young mice from each treatment group, 6 older vehicle and 9 older RTL mice. ^* Indicates p < 0.05 and ^** indicates p < 0.01 compared with vehicle group by t-test.