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. 2014 Sep 25;8:284. doi: 10.3389/fncel.2014.00284

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Copyright © 2014 Dotson, Zhu, Libal, Alkayed and Offner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of RTL1000 on peripheral immune subsets in younger vs. older mice. Spleens were harvested 96 h after MCAO and immunophenotyped by flow cytometry. The frequency of CD4+ T cells (A), CD8+ T cells (B), CD3+ T cells (C), CD11b+ monocytes/neutrophils (D), CD11b+Ly6C+ monocytes/macrophages (E), and CD19+ B cells (F) were determined. Values represent mean numbers (±s.e.m.) of 5 young mice from each treatment group, 4–6 older vehicle and 9 older RTL mice. ^* Indicates p < 0.05, ^** indicates p < 0.01, and ^*** indicates p < 0.001 compared with vehicle group by t-test.