Figure 7.

Inflammatory cytokines are elevated in spleens from older mice after MCAO. Spleens were harvested 96 h after MCAO. Representative flow cytometry dot plots of IFNγ (A) and TNFα (B) producing immune subsets. IFNγ production was determined by gating on CD4+ (C) or CD8+ (D) subsets and measuring IFNγ positive cells compared to isotype. TNFα production was determined by gating on CD4+ (E) or CD11b+ (F) subsets and measuring TNFα positive cells compared to isotype. Values represent mean numbers (±s.e.m.) of 5 young mice from each treatment group, 3 older vehicle and 5 older RTL mice. ^* Indicates p < 0.05 and ^*** indicates p < 0.001 compared with vehicle group by t-test.