Fig. 5.

Influence of root treatment with chemicals present in n-butanol subfraction 3-1 on the number of symptomatic leaves on tobacco after inoculation with soft rot pathogen P. carotovorum. Sterile distilled water (SDW) and 0.5 mM Benzothiadiazole (BTH) were used as negative and positive control, respectively. The highest ISR eliciting subfraction 3–1 was subjected to GC/ MS analysis and chemicals present were purchased and subjected to ISR bioassay. The data are representative of three experiments, three replicates per treatment with 24 plants per replicate. Blank bars mean plants died after treatment. Vertical bar represents standard errors. *Indicates significantly different from negative control at LSD (p = 0.05).