Table 3.
Primers used in genetic diagnosis for the specimens of red pepper infecting viruses majored in Korea
| Virusa | Primerb | Sequences | Size (nt) |
|---|---|---|---|
| BBWV2 | BBWV2 1u | 5′-AAACAAACAGCTTTCGTTCCG-3′ | 380 |
| BBWV2 1R | 5′-GCCATCTCATTGGCATGGA-3′ | ||
|
| |||
| CMV | CMV 3-3u | 5′-AAGAARCTTGTTTCGCGCATT-3′ | 629 |
| CMV 3-3d | 5′-TGGTCTCCTTTTRAGGCCCCCA-3′ | ||
|
| |||
| PMMoV | PMMoV u6 | 5′-TAAATGGCGTTAGTAGTCAAGGA-3′ | 1451 |
| PMMoV d6 | 5′-TGGGCCGCTACCCGCGGTTCGGGG-3′ | ||
|
| |||
| PepMoV | PepMoV u1 | 5′-AATGGCACGTCCCCAAA-3′ | 705 |
| PepMoV d1 | 5′-TCTCTCTCATGCCAACTACGA-3′ | ||
|
| |||
| PVY | PVY u1 | 5′-TGGTGCATTGARAATGGAACCTC-3′ | 755 |
| PVY d1 | 5′-GTCTCCTGATTGAAGTTTACAAG-3′ | ||
a
BBWV2, Broad bean wilt virus 2; CMV, Cucumber mosaic virus; PMMoV, Pepper mild mottle virus; PepMoV, Pepper mottle virus; PVY, Potato virus Y.
b
Primers were designed based on the nucleotide sequences of pepper viruses registered in GenBank.