Table 5.
Group-specific primer pairs for BBWV2 RNA-1 and RNA-2 RNA
| RNA | Group | Primer | Sequences(5′ → 3′)b | Position | Size (nt) |
|---|---|---|---|---|---|
| 1 | I | BBWV2 1-7F | GGAGGGATGTTTTACCAGCT | 4476–5252 | 776 |
| BBWV2 1-7R | CTATTGCGGAATTCCTTGGC | ||||
|
| |||||
| II | BBWV2 1-5f | GATGTGGAAACTTTGCCCAA | 3495–4390 | 895 | |
| BBWV2 1-5r | TTGCTCACTTCTTCAAACCAAG | ||||
|
| |||||
| 2 | I | BBWV2-R2-F | ARTTGGYAAAGGTAARGCGCA | 2731–3226 | 495 |
| BBWV2-R2-R | GCCATCMACTGGRCCTACAACC | ||||
|
| |||||
| II | BBWV2-r2-f | CATAGGGAAGGYAARACTATA | |||
| BBWV2-r2-r | ACCATCCACGGGACCAACCACT | ||||
a
BBWV2, Broad bean wilt virus 2.
b
The primers were selected after alignment of the nucleotide sequences of BBWV2 isolates registered in GenBank.