Table 1.
Primers used for RT-PCR and RT-qPCR analyses
| Gene (2-DE spot) | Forward primer (5′-3′) Reverse primer (5′-3′) |
aTa (°C) | Amplicon size (bp) |
|---|---|---|---|
| omp (spot 14) | F: ACTCGTGGCTCTACTACG | 50 | 381 |
| R: ATGCGTTGTTATACAGG | |||
|
| |||
| omp (spot 15) | F: ATCACGGCACAAGACGC | 60 | 255 |
| R: AGCGTTCTGAGCAGCGAACG | |||
|
|
|||
| for RT-qPCR | F: GCGCTGGCAACCTGCGTTCG | 60 | 166 |
| R: GTGCGCTTGGACAGCGCGTA | |||
|
| |||
| rps (spot 16) | F: AAGCGCCAGTCGCGTGAGATCC | 60 | 407 |
| R: TTGCTCGAGTCGTCGTTACC | |||
|
| |||
| dps (spot 17) | F: TCAAGACCCACAACTTCC | 54 | 249 |
| R: TGACGGACTCGTGACCG | |||
|
|
|||
| for RT-qPCR | F: GGACCGCCGTGGATTCGGTGG | 60 | 189 |
| R: CCGGGAACAGGCTGCGTGCG | |||
|
| |||
| epsD (spot 18) | F: TGCTGGAATCCACTTCG | 50 | 360 |
| R: TCTCATCACAGATCATCG | |||
a
Annealing temperature for PCR amplification