Fig. 5.

Transcript accumulations from genes, AtNR1, AtNR2 and AtNOS1, encoding proteins involved in NO synthesis. Gene expression was determined with RNA extracted from plants without roots after seedlings were treated with 100 μM 2R,3R-butanediol (2,3-B). Gene expression was analyzed by real-time RT-PCR in RNA extracted from Arabidopsis seedling that its roots treated with 100 μM 2R,3R-butanediol for 0, 12, 24, 48, and 72 h. Specific primer sets were used to detect each gene expression pattern. Data were normalized based on actin gene expression. Vertical bars indicate ± standard deviation of three independent experiments with three plants/treatment. Different letters indicate significant difference between treatments according to the least significant differences test at P < 0.05.