Fig. 1.

Construction of full-length monomeric cDNA clones of CSVd-SK1. (A) Schematic sequences of both vector and cDNA insert of CSVd-SK1 in pGEM-CSVd-SK1. SP6 promoter site (red and underline) and SacII site (blue) were used for the synthesis of positive-sense transcript of CSVd-SK1. T7 promoter site (black and underline) and SpeI site (blue) were used for the production of negative-sense transcript of CSVd-SK1. The CSVd-SK1 cDNA is presented as a box. (B) Schematic nucleotide sequence of the cDNA insert of CSVd-SK1 in pCSVd-SK1. SP6 promoter site (red and underline) and EcoRI site (blue) were used for the synthesis of positive-sense transcript of CSVd-SK1. T7 promoter site (black and underline) and XbaI site (blue) were used for the production of negative-sense transcript of CSVd-SK1. SP6 and T7 Transcription start sites are indicated by rectangle arrows.