Fig. 2.

RT-PCR analysis of transcripts from the cDNA clones of CSVd-SK1 in chrysanthemum and tomato plants. Total RNAs were extracted from upper (non-inoculated) leaves of chrysanthemum and tomato plants. RT-PCR was analyzed with CSVd-specific primers [CSVd-For: 5′-aaagaaatgaggcgaagaag-3′ and CSVd-Rev: 5′-ttctttcaaagcagcagggt-3′], as described previously (Chung et al., 2005). The CSVd-SK1 RNAs purified from the infected chrysanthemum plant were used as a positive control and total RNA from mock inoculation (only with 0.5X PBS buffer) was used as a negative control. RT-PCR products were visualized in 1.2% agarose gel.