Table 1.
Primers used in this study
| Clone | 5′-Oligo | 5′-Oligo sequence | 3′-Oligo | 3′-Oligo sequence | Feature |
|---|---|---|---|---|---|
| Primers used for yeast two hybrid assay | |||||
| AltMV TGB1 | TGB1_EcoRI_F | GAGGAATTCATGAAT-CACTTACTAACC | TGB1_BamHI _R | GAGGGATCCCCCGAGTA-ATCGGCGGGG | EcoRI and BamHI |
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| Primers used for BiFC assay | |||||
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| AltMV TGB1 | TGB1_XhoI_F | GAGCTCGAGATGAAT-CACTTACTAACC | TGB1_XmaI _R | GAGCCCGGGCCCGAGTA-ATCGGCGGGG | XhoI and XmaI |
| A. thaliana delta | At_delta_XhoI _F | GAGCTCGAGATGTTTAAA-CAAGCTTCTC | At_delta_XmaI_R | GAGCCCGGGCCCGCCC-GAGAGAGCTGC | XhoI and XmaI |
| A. thaliana LHCA4 | At_LHCA4_XhoI _F | GAGCTCGAGATGGCTACT-GTCACTACTC | At_LHCA4_XmaI_R | GAGCCCGGGCCCGTT-GAAGGTTTGGAC | XhoI and XmaI |
| A. thaliana LHB1B2 | At_LHB1B2_XhoI _F | GAGCTCGAGATG-GCTTCCTCAACCATGG | At_LHB1B2_XmaI_R | GAGCCCGGGCCCCTTTCC-GGGGACGAAG | XhoI and XmaI |
| A. thaliana ATCPIS-CA | At_ATCPISCA_XhoI _F | GAGCTCGAGATGGCTTTC-GCTACTGG | At_ATCPISCA_XmaI_R | GAGCCCGGGCCCCATCTC-GGCAGC | XhoI and XmaI |
| N. benthamiana alpha ATPase | Nb_ATPase_alpha_SalI _F | GAGGTCGACATGAGAAT-CAATCCTAC | Nb_ATPase_alpha_XhoI_R | GAGCTCGAGCCCTTTCTT-CAAATTGCTC | SalI and XhoI |
| N. benthamiana beta ATPase | Nb_ATPase_beta_SalI _F | GAGGTCGACATGGTAAC-CATTCGAGCTGACC | Nb_ATPase_beta_XhoI_R | GAGCTCGAGCCCTGCTT-GTTCCTGAAG | SalI and XhoI |
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| Primers used for TRV VIGS | |||||
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| N. benthamiana beta ATPase silencing | Nb_beta_silencing_R | GAGGGATCCATGGTAAC-CATTCGAGCTGAC | Nb_beta_silencing_R | GAGCTCGAGCACGGG-TATCTGAGCAATTCT | BamHI and XhoI |
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| Primers used for QRT-PCR | |||||
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| QRT-PCR N. ben-thamiana Actin | QRT_Nb_Actin_F | ATTGTCAG-CAACTGGGATG | QRT_Nb_Actin_R | ATTGTCAGCAACTGGGATG | |
| QRT-PCR TRV Coat Protein | QRT_Nb_ATPase_beta_F | TGGGAGATATGTACGAT-GAATC | QRT_Nb_TRV_CP_R | GCTGTGTAACTTCTTCAA-CAA | |
| QRT-PCR N. ben-thamiana beta ATPase | Nb_beta_silencing_F | TGGCAAGAGGTCAACGAT | QRT_Nb_ATPase_beta_R | CCTGTCCAACTTCTAAT-GAATCA | |