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Selected primer sets for RT-PCR and nested PCR of PDV
| Primer
|
Sequence | Length (bp) | Product size (bp) | ||
|---|---|---|---|---|---|
| Set | PCR | Name | |||
| 7 | RT | PDV-F20 | ACGATTGGTTAACTCACTTTGT | 22 | 739 |
| PDV-C10 | ATCCACTGACTATTYTATCCATYG | 24 | |||
| Nested | PDV-F60 | AGCTCGGAAGAATAATAATACTAC | 24 | 305 | |
| PDV-C80 | CGAAACCTTTAATGAGTCCGTAGA | 24 | |||
|
| |||||
| 27 | RT | PDV-F10 | GAAGCTTTTGGTGTAACGATTGGT | 24 | 673 |
| PDV-C20 | TCTTTGGCATCGAGTGTTGG | 20 | |||
| Nested | PDV-F60 | AGCTCGGAAGAATAATAATACTAC | 24 | 305 | |
| PDV-C80 | CGAAACCTTTAATGAGTCCGTAGA | 24 | |||
