Fig. 3.

A gacS mutation affects fleQ, fliQ and flhF transcript accumulation in Pseudomonas chlororaphis O6. Reverse transcription-polymerase chain reaction (RT-PCR) reactions were stopped at the end of the 15th cycle, and PCR products were loaded on a 2.0% agarose gel. The intensity of the PCR products was used to measure relative transcript accumulations in cells of the wild type, the gacS mutant (GacS-), and the complemented GacS mutant (ComGacS-). Expression from the 16S rRNA genes was used to standardize transcript levels. Relative expression of the genes in P. chlororaphis O6 strains are means of three independent experiments, and different letters indicate significant differences in gene expression among P. chloraphis O6 strains according to Duncan’s analysis of variance test (P < 0.05).