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Transcript accumulations from selected genes in the wild type (O6), the gacS mutant (GacS-) and the complemented gacS mutant (cGacS-) were assessed by RT-PCR. Data are shown for RNA extracted from stationary phase cells from one of two independent studies showing the same results. PCR bands from RNA transcripts of the 16S rRNA genes are shown to confirm equal loading of the wells.
