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Auxotrophic phenotype of Pseudomonas chlororaphis O6 trpE mutant under minimal growth conditions. Each bacterial strain O6 (wild type), the gacS mutant (GacS-), the complemented gacS mutant (cGacS-), and the trpE mutant (TrpE-) was applied to Luria Bertani agar (LB), M9 minimal agar (M9), or M9 minimal agar with 5 mM tryptophan. The growth images were photographed two days after inoculation on the plates. The images are representative of three independent experiments.
