Fig. 5.

Effect of mutations in prnA and trpE in Pseudomonas chlororaphis O6 on colonization of roots of tobacco under sterile, non-competitive growth conditions. Three week-old tobacco roots in microtiter plates were inoculated with the wild type strain (Wt), the prnA mutant (PrnA-) and trpE mutant (TrpE-) of P. chlororaphis O6. At the defined days after inoculation, root colonization of each bacterial strain was measured based on culturable cells obtained from excised roots. Data are the means of three independent studies with three plants per treatment in each study. Vertical bars represent standard errors. * indicates differences between bacterial strains by Duncan’s multiple range test at p< 0.05.