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. 2014 Sep 25;8:101. doi: 10.3389/fnana.2014.00101

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Copyright © 2014 Cavaliere, Ghirardi and Tirindelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ESP1-immunoreactivity in the tear fluid after removal of the extraorbital lacrimal glands. (A) The surgical area after the incision of the skin in the temporal region of the mouse is photographed. Shown are the extraorbital gland (ELG), the intraorbital gland (ILG), the lacrimal duct and the superficial temporal vein (STV). (B) The lacrimal fluid of seven BALB/c male mice, which underwent the removal of ELGs (ELGx), and of seven sham operated mice (control) was individually collected and analyzed by western blotting with an antibody against ESP1. Tear fluids were also pooled from five BALB/c female mice and used as a negative control. Arrowhead indicates the position of the molecular marker (aprotinin, 6.5 KDa).