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. 2014 Sep 25;8:101. doi: 10.3389/fnana.2014.00101

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Copyright © 2014 Cavaliere, Ghirardi and Tirindelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ESP1 expression in lacrimal glands and tear fluid. (A) Soluble lacrimal gland extracts of BALB/c mice analyzed for ESP1 immunoreactivity by western blotting. Arrowhead indicates the position of the molecular marker (aprotinin, 6.5 KDa). (B) Expression pattern of the ESP1 gene in different lacrimal glands of BALB/c males, as determined by RT–PCR. PCR control without cDNA is also shown. Arrowhead indicates the position of the 500 bp DNA marker. (C) ESP1 immunoreactivity in tear fluids, analyzed by western blotting, in five BALB/c males after the removal of both ELGs and ILGs (ELGx/ILGx). Tear fluids of positive (BALB/c males) and negative (BALB/c females) controls were also tested for ESP1 immunoreactivity. Arrowhead indicates the position of the molecular marker (aprotinin, 6.5 KDa).