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. 2014 Sep 25;8:101. doi: 10.3389/fnana.2014.00101

Figure 3.

Figure 3

Removal of the ESP1-expressing lacrimal glands impairs sexual behavior in BALB/c females. (A) BALB/c and C57BL/6 females were exposed to BALB/c males, which were deprived of the extraorbital glands (ELGx) or both the extraorbital and intraorbital glands (ELGx/ILGx), and to sham operated males (control). Histograms represent the number of male mounts, percentage of successful male intromissions per total mounts and percentage of female lordosis response per total mounts. Error bars represent SE; n = 7 for each animal group. * P < 0.05, ** P < 0.01, *** P < 0.0001. (B) ESP1 immunoreactivity in the extra and intraorbital gland soluble extracts of BALB/c and C57BL/6 male mice. (C) ESP1 immunoreactivity in the tear fluid of BALB/c and C57BL/6 male mice. BALB/c lacrimal fluid was diluted 30 and 60 times, respectively and the same volume of solution was loaded. Arrowhead indicates the position of the molecular marker (aprotinin, 6.5 KDa). (D) RT-PCR with ESP1 specific primers of cDNA synthesized from the mRNA of the extraorbital glands of BALB/c and C57BL/6 males. Arrowhead indicates the position of the 500 bp DNA marker.