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. 2014 Aug 20;1(Pt 5):305–317. doi: 10.1107/S2052252514014900

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© Ho-Hsien Lee et al. 2014

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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CTBMPR exists in several metastable oligomeric forms. Affinity-purified CTBMPR was resolved by SEC–HPLC, yielding three major peaks probably corresponding to pentamers (fraction 8) and monomers (fraction 16). Fractions 21 and 23 did not contain appreciable amounts of protein and are likely to contain high concentrations of imidazole. The shoulder at the right of the pentamer peak (fraction 11) may represent the less stable intermediates tetramers and dimers. These fractions (numbered in red), alongside the original sample and a precipitate that formed in the original sample, were analyzed by SDS–PAGE followed by silver staining (inset).